Autoclave Machine: Types, Features, Uses, Prices, and Financing Options in India

Discover autoclave machines, their types, features, uses, pricing in India, and financing options to make the right purchase decision.
Medical Equipment Finance
3 min
07 April 2025

Autoclave machines play a very crucial role in ensuring hygiene and sterility across multiple industries, right from healthcare to pharmaceuticals and research laboratories. These steam sterilisers use high-pressure steam to eliminate bacteria, viruses, fungi, and spores, making them indispensable for preventing contamination. Whether you are looking to purchase an autoclave machine or understand its sterilisation process, keep reading to get valuable insights that will help you make informed decisions.

What is an autoclave machine?

Autoclaves, commonly referred to as steam sterilizers, are essential machines used in both healthcare and industrial settings to ensure thorough sterilisation. These devices operate by generating steam under high pressure to eliminate harmful microorganisms, including bacteria, viruses, fungi, and spores, from various items placed inside the pressure vessel. The process involves heating the items to a specific sterilisation temperature for a predetermined duration. The steam’s moisture plays a crucial role in this procedure, as it efficiently transfers heat to the items, thereby disrupting and destroying the protein structures of the bacteria and spores. This method ensures a high level of cleanliness and safety, making autoclaves indispensable for maintaining hygiene standards and preventing contamination in various applications.

How does an autoclave machine work?

Autoclaves are widely used in healthcare environments to sterilise medical instruments. Items are placed inside a pressure vessel, known as the chamber, where three key factors—time, temperature, and steam quality—are crucial for effective steam sterilisation.

The autoclave process consists of three phases:

  1. Conditioning Phase (C): This initial phase involves removing air from the chamber, as air can hinder sterilisation. In dynamic air removal autoclaves, a vacuum system removes the air, while systems without a vacuum use steam flushes and pressure pulses. Gravity-type sterilizers utilise steam to push the air down and out through the steriliser drain.
  2. Exposure Phase (S): Once the air is expelled, the steriliser drain closes, and steam is continuously introduced into the chamber, raising both pressure and temperature to the required levels. The items are held at this sterilisation temperature for a set time to ensure thorough sterilisation.
  3. Exhaust Phase (E): In the final phase, the steriliser drain opens to release the steam, which depressurises the chamber and allows the items to dry.

For effective sterilisation, the steam must be of high quality, consisting of 97% steam (vapour) and 3% moisture (liquid water). This composition ensures optimal heat transfer. Steam with less than 3% moisture is considered superheated and is less effective for sterilisation due to inefficient heat transfer.

Types of autoclave machines

Introducing the various types of autoclave machines, this concise guide highlights the versatility and specialised functions of each type. Understanding these distinctions allows for informed decisions regarding the most suitable autoclave for specific sterilisation needs.

  • Gravity autoclave: Utilises gravity to remove air from the chamber before steam enters, ideal for sterilising liquids, media, and glassware.
  • Pre-vacuum autoclave: Employs a vacuum pump to remove air from the chamber before steam injection, suitable for sterilising solid items and porous loads.
  • Steam Flush Pressure Pulse (SFPP) autoclave: Utilises a series of steam pulses to achieve sterilisation, ideal for sterilising delicate instruments and materials.
  • Positive pressure autoclave: Maintains positive pressure throughout the cycle, preventing air re-entry and ensuring effective sterilisation of wrapped instruments.
  • Negative pressure autoclave: Operates under negative pressure, suitable for sterilising biohazardous waste and ensuring containment of potentially harmful materials.

Each type of autoclave machine offers unique advantages and is tailored to specific sterilisation requirements, providing flexibility and efficiency in diverse applications.

Features and specifications of autoclave machines

Autoclave machines come with essential features and specifications, ensuring optimal functionality:

  • Precision controls: Facilitate accurate adjustments.
  • Safety mechanisms: Guarantee secure operations.
  • Efficient sterilization: Ensuring thorough disinfection.

Uses of autoclave machine

The autoclave machine finds widespread applications across various industries due to its ability to effectively sterilise equipment and materials.

  1. Healthcare: Autoclaves are crucial in healthcare facilities for sterilising surgical instruments, medical devices, and laboratory equipment, effectively eliminating harmful microorganisms and preventing the spread of infections.
  2. Pharmaceuticals: Pharmaceutical companies rely on autoclaves to sterilise drug formulations, containers, and production equipment, ensuring product integrity and safety.
  3. Food industry: Autoclaves play a vital role in the food industry by sterilising canned foods, effectively eliminating pathogens and extending shelf life while maintaining product quality.
  4. Research laboratories: Autoclaves are utilised in research laboratories for sterilising media, glassware, and experimental setups, ensuring sterile conditions and reliable experimental results.
  5. Versatility: The versatility and reliability of autoclave machines make them indispensable tools in maintaining hygiene and safety standards across various sectors.

Difference between aluminium and stainless steel vertical autoclave machines

When selecting between aluminium and stainless steel vertical autoclaves, consider the following key differences to determine which material best suits your usage needs:

Feature

Stainless steel autoclave

Aluminium autoclave

Durability

Highly durable and built for long-term use

Less durable, suitable for light to moderate use

Corrosion resistance

Excellent resistance to rust and corrosion

Moderate resistance; may corrode over time

Maintenance

Requires minimal maintenance

May require more frequent cleaning or care

Ideal for

Hospitals, laboratories, high-demand applications

Small clinics, portable or cost-sensitive setups

Weight

Heavier and less portable

Lightweight and easy to handle

Cost

Higher upfront investment

Generally more budget-friendly

Reliability in harsh use

Performs well under intensive usage

Best for routine or limited usage

 

Price of autoclave machine in India

Shop with a wide selection of autoclave machines from reputable brands like JE MediGuard, AmbyGO, and Max Life, all known for their reliability and high performance. The prices for these autoclaves vary according to brand, type, and size, with options ranging from ₹5,500 to ₹95,000. However, it is important to note that prices are subject to change.

S. No.

Autoclave Machine Products

Price

1

JE Mediguard 30ltr Wing Nut Type Autoclave Machine

Rs. 12,999

2

Mediguard Electric Autoclave Machine - 21ltr

Rs. 6,199

3

Surgix Autoclave Electric Aluminium Cooker Type

Rs. 10,827

4

Mediguard 30ltr Cooker Type Autoclave Machine

Rs. 9,912

5

JE Mediguard 75ltr Wing Nut Type Autoclave Machine

Rs. 42,499

6

Electric Vertical Autoclave SURGIX Size 16" x 24"

Rs. 56,600

7

JE Mediguard 27ltr Wing Nut Type Autoclave Machine with Timer

Rs. 13,599

 

Choosing the right autoclave machine for your needs

Autoclaves are available in two primary orientations: vertical and horizontal, with each type suited to different sterilisation needs based on the items being loaded.

Vertical autoclave: Also known as a top-loading steriliser, the vertical autoclave features a cylindrical design with the door located at the top of the chamber. This setup is ideal for sterilising items that are easy to load and unload from above, such as laboratory glassware or small instruments. Vertical autoclaves are commonly used in laboratories, smaller clinics, and settings where space is limited, as their compact design requires less floor space.

Horizontal autoclave: In contrast, the horizontal autoclave has a chamber that is set up horizontally, with the door on one side and the opposite wall on the other. This design is well-suited for sterilising larger or bulkier items, such as surgical instruments, medical waste, and large batches of equipment. Horizontal autoclaves are typically found in hospitals, large research facilities, and industrial settings where high-volume sterilisation is required.

The choice between vertical and horizontal autoclaves depends largely on the specific needs of the facility, including the types of items being sterilised, available space, and workflow requirements.

Things to keep in mind while purchasing an autoclave machine

  1. Understand the intended use and choose a machine that aligns with those requirements.
  2. Ensure the autoclave fits the designated workspace in terms of size and specifications.
  3. Evaluate maintenance needs; some machines may require more attention than others.
  4. Inquire about warranty terms and assess safety features.
  5. Consider the reputation of the manufacturer.
  6. Keeping these factors in mind ensures a well-informed decision that meets both operational needs and long-term reliability.

Financing options for autoclave machine purchase

Explore convenient financing options, such as a medical equipment loan to facilitate the acquisition of autoclave machines.

Conclusion

In conclusion, autoclave machines are indispensable for maintaining sterile environments. Understanding their types, operations, features, and financing options is crucial in making informed decisions. For medical professionals exploring financial support, consider a doctor loan for comprehensive assistance in acquiring essential equipment.

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Frequently asked questions

What is the use of an autoclave machine?

An autoclave machine is primarily used for sterilization in medical and laboratory settings. Its high-pressure and high-temperature environment effectively eliminates bacteria, viruses, and other microorganisms from instruments and equipment, ensuring aseptic conditions for medical procedures and experiments.

What is the principle of autoclave?

The fundamental principle of an autoclave revolves around steam sterilisation. By leveraging high-pressure saturated steam at a temperature of around 121 degrees Celsius or more, an autoclave efficiently kills bacteria, viruses, fungi, and spores. The procedure typically lasts around 15 to 20 minutes, depending on the volume and the nature of the material. This courtesy of the moist heat produced, which coagulates the proteins, causing the microorganisms to perish.

What are the advantages of autoclave?

The autoclave exhibits a range of advantages, particularly in healthcare settings. Its ability to thoroughly sterilise equipment and materials is notably essential in preventing the spread of infections in medical environments. This equipment can sterilise a wide variety of heat-resistant items, covering everything from surgical instruments to glassware. Also, the use of an autoclave is quite straightforward and doesn't necessitate any intricate training. The autoclave also provides thorough sterilisation without the use of harmful chemicals that could remain on the sterilised items and potentially cause harm. Lastly, the rapid and effective nature of autoclaves combined with their relative affordability makes them an excellent tool for many fields, especially those related to healthcare and laboratory work.

What are the common troubleshooting steps for an autoclave?

Common troubleshooting steps for an autoclave include:

  1. Check power supply: Ensure the autoclave is properly plugged in and receiving power.
  2. Inspect door seal: Look for cracks or damage in the gasket, which can cause steam leaks.
  3. Verify water levels: Ensure the water reservoir is filled to the appropriate level.
  4. Monitor temperature and pressure gauges: Confirm they reach the required levels for sterilisation.
  5. Clean filters and chambers: Regularly clean to prevent blockages.
  6. Reset the machine: If issues persist, try resetting the autoclave according to the manufacturer's instructions.
What does an autoclave machine do?

An autoclave machine sterilises medical and laboratory equipment using high-pressure steam. It eliminates bacteria, viruses, fungi, and spores by heating items to 121°C–134°C inside a sealed chamber. Commonly used in hospitals and labs, it ensures tools are safe for reuse and maintains hygiene in clinical environments.

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