Autoclave Machine: Definition, Component, Types, Procedure, Uses, Guidelines, Prices and Financing Options

Discover a comprehensive guide to autoclave machines, their types, features & pricing. Also explore financing options and valuable tips for a smart autoclave purchase.
Medical Equipment Finance
3 min
02-September-2024

Discovering the world of sterilization is simplified with autoclave machines. These devices play a pivotal role in ensuring the cleanliness and safety of various tools and instruments in medical and laboratory settings. Let's explore the basics, types, working mechanisms, features, pricing, and essential considerations when delving into the world of autoclaves.

What is an autoclave machine?

Autoclaves, commonly referred to as steam sterilizers, are essential machines used in both healthcare and industrial settings to ensure thorough sterilisation. These devices operate by generating steam under high pressure to eliminate harmful microorganisms, including bacteria, viruses, fungi, and spores, from various items placed inside the pressure vessel. The process involves heating the items to a specific sterilisation temperature for a predetermined duration. The steam’s moisture plays a crucial role in this procedure, as it efficiently transfers heat to the items, thereby disrupting and destroying the protein structures of the bacteria and spores. This method ensures a high level of cleanliness and safety, making autoclaves indispensable for maintaining hygiene standards and preventing contamination in various applications.

Autoclave Parts/ Components

Understanding the key components of an autoclave is essential for maintaining its functionality and optimising the sterilisation process. Let us delve into the essential parts and components that make autoclaves such vital assets in the pursuit of cleanliness and safety.

  • Chamber: The main compartment is where items to be sterilised are placed.
  • Door: Provides access to the chamber and seals it during operation.
  • Control panel: Interface for setting and monitoring temperature, pressure, and cycle duration.
  • Heating element: Generates steam to achieve sterilisation temperature.
  • Pressure gauge: Indicates the pressure inside the chamber.
  • Temperature sensor: Monitors the temperature to ensure effective sterilisation.
  • Safety valves: Release excess pressure to prevent chamber Overpressurisation.
  • Water reservoir: Stores water that is used to generate steam.
  • Drainage system: Removes condensate and excess water after sterilisation.
  • Insulation: Surrounds the chamber to maintain temperature and reduce heat loss.
  • Seals and gaskets: Ensure a tight seal between the door and chamber to prevent steam leakage.
  • Air removal system: Removes air from the chamber to facilitate steam penetration and sterilisation.
  • Rack or tray: Supports items inside the chamber during sterilisation, allowing for proper steam circulation.

How many types of autoclave machine?

Introducing the various types of autoclave machines, this concise guide highlights the versatility and specialised functions of each type. Understanding these distinctions allows for informed decisions regarding the most suitable autoclave for specific sterilisation needs.

  • Gravity autoclave: Utilises gravity to remove air from the chamber before steam enters, ideal for sterilising liquids, media, and glassware.
  • Pre-vacuum autoclave: Employs a vacuum pump to remove air from the chamber before steam injection, suitable for sterilising solid items and porous loads.
  • Steam Flush Pressure Pulse (SFPP) autoclave: Utilises a series of steam pulses to achieve sterilisation, ideal for sterilising delicate instruments and materials.
  • Positive pressure autoclave: Maintains positive pressure throughout the cycle, preventing air re-entry and ensuring effective sterilisation of wrapped instruments.
  • Negative pressure autoclave: Operates under negative pressure, suitable for sterilising biohazardous waste and ensuring containment of potentially harmful materials.

Each type of autoclave machine offers unique advantages and is tailored to specific sterilisation requirements, providing flexibility and efficiency in diverse applications.

How does an autoclave work?

Autoclaves are widely used in healthcare environments to sterilise medical instruments. Items are placed inside a pressure vessel, known as the chamber, where three key factors—time, temperature, and steam quality—are crucial for effective steam sterilisation.

The autoclave process consists of three phases:

  1. Conditioning Phase (C): This initial phase involves removing air from the chamber, as air can hinder sterilisation. In dynamic air removal autoclaves, a vacuum system removes the air, while systems without a vacuum use steam flushes and pressure pulses. Gravity-type sterilizers utilise steam to push the air down and out through the steriliser drain.
  2. Exposure Phase (S): Once the air is expelled, the steriliser drain closes, and steam is continuously introduced into the chamber, raising both pressure and temperature to the required levels. The items are held at this sterilisation temperature for a set time to ensure thorough sterilisation.
  3. Exhaust Phase (E): In the final phase, the steriliser drain opens to release the steam, which depressurises the chamber and allows the items to dry.

For effective sterilisation, the steam must be of high quality, consisting of 97% steam (vapour) and 3% moisture (liquid water). This composition ensures optimal heat transfer. Steam with less than 3% moisture is considered superheated and is less effective for sterilisation due to inefficient heat transfer.

Features and specifications

Autoclave machines come with essential features and specifications, ensuring optimal functionality:

  • Precision controls: Facilitate accurate adjustments.
  • Safety mechanisms: Guarantee secure operations.
  • Efficient sterilization: Ensuring thorough disinfection.

Procedure for running an autoclave machine

By following these carefully outlined pointers, users can navigate the operation of an autoclave with confidence, ensuring both safety and efficacy in the sterilisation process.

  • Prepare items: Load the items to be sterilised into the chamber, ensuring proper arrangement for steam circulation.
  • Fill water reservoir: Add water to the autoclave's reservoir to generate steam during the sterilisation process.
  • Check drainage system: Ensure that the drainage system is clear to allow proper removal of condensate and excess water.
  • Secure door: Close the autoclave door securely, ensuring the seals are intact to prevent steam leakage.
  • Set parameters: Use the control panel to set sterilisation parameters such as temperature, pressure, and cycle duration.
  • Start autoclave: Initiate the autoclave cycle, allowing it to heat up and build pressure according to the preset settings.
  • Monitor cycle: Keep an eye on the autoclave during the sterilisation cycle to ensure it proceeds without issues.
  • Depressurise: Once the cycle is complete, allow the autoclave to depressurise before attempting to open the door.
  • Open door: Carefully open the autoclave door, taking precautions to avoid burns from hot surfaces.
  • Remove items: Safely remove sterilised items from the chamber, using appropriate protective equipment if necessary.
  • Drain chamber: Drain any remaining water from the autoclave chamber and clean it as needed for future use.

Following these steps ensures a thorough sterilisation process and safe operation of the autoclave.

Uses of Autoclave Machine

The autoclave machine finds widespread applications across various industries due to its ability to effectively sterilise equipment and materials.

  1. Healthcare: Autoclaves are crucial in healthcare facilities for sterilising surgical instruments, medical devices, and laboratory equipment, effectively eliminating harmful microorganisms and preventing the spread of infections.
  2. Pharmaceuticals: Pharmaceutical companies rely on autoclaves to sterilise drug formulations, containers, and production equipment, ensuring product integrity and safety.
  3. Food industry: Autoclaves play a vital role in the food industry by sterilising canned foods, effectively eliminating pathogens and extending shelf life while maintaining product quality.
  4. Research laboratories: Autoclaves are utilised in research laboratories for sterilising media, glassware, and experimental setups, ensuring sterile conditions and reliable experimental results.
  5. Versatility: The versatility and reliability of autoclave machines make them indispensable tools in maintaining hygiene and safety standards across various sectors.

Precautions

When operating an autoclave machine, several precautions must be observed to ensure safety and optimal performance.

  1. Training and familiarisation: Adequate training and understanding of the autoclave's operation manual are crucial to prevent accidents and equipment damage.
  2. Adherence to instructions: Following manufacturer guidelines regarding load capacity, sterilisation parameters, and cycle times is essential for safe and effective operation.
  3. Personal protective equipment (PPE): Wearing appropriate PPE such as gloves and safety goggles protects against burns and exposure to steam during operation.
  4. Maintenance and calibration: Regular maintenance and calibration of the autoclave ensure consistent sterilisation efficacy and prevent malfunctions.
  5. Inspection: Before each use, operators should inspect seals, valves, and pressure gauges to ensure proper functioning and safety.
  6. entilation and cooling: Proper ventilation of the autoclave chamber and allowing adequate cooling time after sterilisation cycles are crucial to prevent steam burns and ensure safe handling of sterilised items.

Advantages of autoclaves

  1. Effective sterilisation: Autoclaves utilise high-pressure steam to reach temperatures exceeding 250°F (121°C), effectively eliminating all microorganisms. This method is ideal for materials that cannot be sterilised by dry heat, such as items containing moisture.
  2. Versatility: Autoclaves are capable of sterilising a wide range of materials, including solids, liquids, glassware, and specialised laboratory tools. This adaptability makes them crucial in both medical and laboratory environments.
  3. Safety features: Modern autoclaves come with built-in safety mechanisms to prevent excessive pressure and include systems that cool water before discharge, thus safeguarding the facility's infrastructure.
  4. Standardisation: Standardised sterilisation cycles, whether gravity or liquid-based, ensure consistent and reliable results. This uniformity guarantees that all items undergo the same level of treatment, enhancing overall reliability.

Disadvantages of an autoclave

  1. Unsuitable for heat-sensitive materials: Autoclaves are ineffective for sterilising heat-sensitive items, such as certain plastics, fabrics, and high-protein solutions. The intense heat can cause these materials to degrade or melt.
  2. Risk to sharp instruments: The high temperatures and steam in autoclaves can dull the edges of sharp instruments like scissors and scalpel blades, leading to reduced performance and the need for more frequent replacements.
  3. Restrictions for specific substances: Autoclaves cannot effectively sterilise oily substances or certain chemical compounds, as these materials do not interact well with water or withstand high heat.
  4. Operational challenges: Using an autoclave requires strict adherence to operational protocols and regulatory standards, demanding trained personnel and careful monitoring to ensure proper function.

In summary, while autoclaves are highly effective and versatile for many sterilisation tasks, they have limitations regarding certain materials and can cause damage to specific items. Selecting the appropriate sterilisation method based on material properties is essential for achieving the best results.

Comparison between aluminium and stainless steel vertical autoclaves

When choosing between aluminium and stainless steel for vertical autoclave machines, it’s essential to consider your specific requirements. Stainless steel is often favoured for its superior durability, resistance to corrosion, and low maintenance, making it ideal for high-demand settings such as hospitals and research laboratories. Its robust nature ensures long-term reliability, even in rigorous environments. On the other hand, aluminium can be a suitable option for smaller-scale applications where the weight and cost are key factors. Aluminium is lighter and often more affordable, making it practical for less demanding tasks or where portability is needed. Ultimately, the decision depends on the balance between the durability and strength of stainless steel versus the lightweight and cost-effectiveness of aluminium, tailored to your specific use case.

Pricing information

Understanding the pricing of autoclave machines involves evaluating factors like type, size, features, brand, material quality, accessories, installation, and maintenance costs. Larger capacities, advanced features, and reputable brands typically contribute to higher prices. The quality of materials, additional accessories, and customization options can also impact costs. Consideration of installation expenses, ongoing maintenance requirements, and market trends is essential. Buyers should assess their specific needs and budget constraints, comparing prices and features to make an informed decision that aligns with both functional requirements and financial considerations.

Purchase autoclave at affordable price on hospital store

Shop with a wide selection of autoclave machines from reputable brands like JE MediGuard, AmbyGO, and Max Life, all known for their reliability and high performance. The prices for these autoclaves vary according to brand, type, and size, with options ranging from ₹5,500 to ₹95,000. However, it is important to note that prices are subject to change.

S. No.

Autoclave Machine Products

Price

1

JE Mediguard 30ltr Wing Nut Type Autoclave Machine

₹ 12,999

2

Mediguard Electric Autoclave Machine - 21ltr

₹ 6,199

3

Surgix Autoclave Electric Aluminium Cooker Type

₹ 10,827

4

Mediguard 30ltr Cooker Type Autoclave Machine

₹ 9,912

5

JE Mediguard 75ltr Wing Nut Type Autoclave Machine

₹ 42,499

6

Electric Vertical Autoclave SURGIX Size 16" x 24"

₹ 56,600

7

JE Mediguard 27ltr Wing Nut Type Autoclave Machine with Timer

₹ 13,599

 

Buy autoclave according to orientations

Autoclaves are available in two primary orientations: vertical and horizontal, with each type suited to different sterilisation needs based on the items being loaded.

Vertical autoclave: Also known as a top-loading steriliser, the vertical autoclave features a cylindrical design with the door located at the top of the chamber. This setup is ideal for sterilising items that are easy to load and unload from above, such as laboratory glassware or small instruments. Vertical autoclaves are commonly used in laboratories, smaller clinics, and settings where space is limited, as their compact design requires less floor space.

Horizontal autoclave: In contrast, the horizontal autoclave has a chamber that is set up horizontally, with the door on one side and the opposite wall on the other. This design is well-suited for sterilising larger or bulkier items, such as surgical instruments, medical waste, and large batches of equipment. Horizontal autoclaves are typically found in hospitals, large research facilities, and industrial settings where high-volume sterilisation is required.

The choice between vertical and horizontal autoclaves depends largely on the specific needs of the facility, including the types of items being sterilised, available space, and workflow requirements.

Things to keep in mind while purchasing an autoclave machine

  1. Understand the intended use and choose a machine that aligns with those requirements.
  2. Ensure the autoclave fits the designated workspace in terms of size and specifications.
  3. Evaluate maintenance needs; some machines may require more attention than others.
  4. Inquire about warranty terms and assess safety features.
  5. Consider the reputation of the manufacturer.
  6. Keeping these factors in mind ensures a well-informed decision that meets both operational needs and long-term reliability.

Financing options for purchase

Explore convenient financing options, such as a medical equipment loan to facilitate the acquisition of autoclave machines.

Conclusion

In conclusion, autoclave machines are indispensable for maintaining sterile environments. Understanding their types, operations, features, and financing options is crucial in making informed decisions. For medical professionals exploring financial support, consider a doctor loan for comprehensive assistance in acquiring essential equipment.

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Frequently asked questions

What is the use of an autoclave machine?

An autoclave machine is primarily used for sterilization in medical and laboratory settings. Its high-pressure and high-temperature environment effectively eliminates bacteria, viruses, and other microorganisms from instruments and equipment, ensuring aseptic conditions for medical procedures and experiments.

What is the principle of autoclave?

The fundamental principle of an autoclave revolves around steam sterilisation. By leveraging high-pressure saturated steam at a temperature of around 121 degrees Celsius or more, an autoclave efficiently kills bacteria, viruses, fungi, and spores. The procedure typically lasts around 15 to 20 minutes, depending on the volume and the nature of the material. This courtesy of the moist heat produced, which coagulates the proteins, causing the microorganisms to perish.

What are the advantages of autoclave?

The autoclave exhibits a range of advantages, particularly in healthcare settings. Its ability to thoroughly sterilise equipment and materials is notably essential in preventing the spread of infections in medical environments. This equipment can sterilise a wide variety of heat-resistant items, covering everything from surgical instruments to glassware. Also, the use of an autoclave is quite straightforward and doesn't necessitate any intricate training. The autoclave also provides thorough sterilisation without the use of harmful chemicals that could remain on the sterilised items and potentially cause harm. Lastly, the rapid and effective nature of autoclaves combined with their relative affordability makes them an excellent tool for many fields, especially those related to healthcare and laboratory work.

What are the common troubleshooting steps for an autoclave?

Common troubleshooting steps for an autoclave include:

  1. Check power supply: Ensure the autoclave is properly plugged in and receiving power.
  2. Inspect door seal: Look for cracks or damage in the gasket, which can cause steam leaks.
  3. Verify water levels: Ensure the water reservoir is filled to the appropriate level.
  4. Monitor temperature and pressure gauges: Confirm they reach the required levels for sterilisation.
  5. Clean filters and chambers: Regularly clean to prevent blockages.
  6. Reset the machine: If issues persist, try resetting the autoclave according to the manufacturer's instructions.
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